AIM : To study the impact of bacterial conjugation through enthrall of factors,coding for antibiotic resistance.
doctrine : From one bacterial strain(donor) to another strain(recipient) is a known particular that occurs in nature with objective of mixing the gene pool. In otherwise asexually reproducing organism, DNA transfer among bacteria is in different mediating ways:
I. Transformation
II. Transduction
III. Conjugation
Conjugation is the most widespread routine of transferring genetic material from one bacterial cellular phone to another. It is a process in which unidirectional transfer of DNA is arbitrate by conjugal plasmid or conjugal transposons requiring cell to cell contacts. It was discovered by Lederberg and Tatum.
Conjugation is best soundless by considering properties of F-factor, which is a small circular plasmid DNA, that poop replicate autonomously in the cell or can shuffle into host chromosome and thus transfer host chromosomal marker. The man-to-man cells with integrated F-factor ar isolated and allowed to form pure colonies. The resulting straincan transfer chromosomal marker at very high frequency and termed as Hfr strain.
INTRODUCTION: The E.coli strain A & B referred to as parental strains.
give A carries an antibiotic resistant gene in F-plasmid coding for tetracycline, while Strain B is detached of any F-factor, but carries streptomycin resistant gene in its chromosome. consequently both the strains grows on medium containing tetracycline or streptomycin to which they are resistant on conjugating the two parental strains, the resulting culture go out be resistant to both the antibiotics, i.e. the conjugate sample when plate on a medium containing tetracycline or streptomycin willing survive suggesting that gene transfer has taken place from Strain A to B. However the parental strain when plated on the uniform medium does not survive as they are sore to either tetracycline or streptomycin.
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